GL Sciences

DNA purification from PCR sample 4 min
DNA purification from agarose gel 9 min

MonoFas DNA Purification Kit Ⅰ extracts the fragment DNA
from the PCR products and agarose gels. It can be applied
for both centrifugation and aspiration methods. Some good
features realized by Monolithic silica Spin column.

<Fratures>
Fast!
DNA purification from PCR samples in 4 minutes.
DNA extraction from agarose gels in 9 minutes.

Small Sample Volume!
No damages on DNA by a strong centrifuge with monolithic technology. Samples can be as small as 10µL and more than 90% recovery rate.

High Nucleic Acid Retention!
The high retentivity of the Monolithic silica ageinst nucleic acid enables high purification and recovery. The wide range of fragment DNA between 35bp-35kB can be purified.

Product Contents
(MonoFas DNA Purification Kit Ⅰ 50 pcs.)

Spin Column 50 pcs

Collection Tube 50 pcs

Bind Buffer A 30 mL

Wash Buffer B 40 mL

Elute Buffer C 10 mL

Operation Manual 1 pcs

Specifications

Items Purification from PCR Extraction from Agarose gel

Time 4 minutes 9 minutes

Maximum DNA Binding Amount 10μg 10μg

Maximum Gel Throughput - 1g

Minimum Elution Volume 10μL 10μL

Column Volume 1mL 1mL

Processable DNA Range 35bp～35kb 35bp～35kb

Recovery Rate >90%(100bp~5kbp) >80%(100bp~5kbp)

>60%(5kb~35kbp) >50%(5kbp~35kbp)

Primer Elimination Ratio 95% -

Purification Comparison by Chromatogram

①Before the Purification from PCR sample
②After the Purification by MonoFas
③After the Purification by competitor A

1g of Agarose Gel can be Extracted by just 1 time

		The maximum throughput of gel slices is 1g. Massive amount of gel slices can be rapidly extracted.
	400bp M:pHY Marker 1:PCR product 2:Extracted from Agarose Gel(MonoFas Ⅰ)

Highly Effective Ligation

		Lane 1:Target gene (ARL1) purified by MonoFas I was integrated into the Vector, then amplified and extracted. Lane 2:Confirming the target gene by restriction and endonuclease treatment. MonoFas I can increase the ligation efficiency as it highly purifies the fragment DNA. Also neither ethanol precipitation nor phenol extraction before the ligation is necessary.
	pUC119(3162bp) ARL1(552bp) M:pHY Marker 1:Plasmid DNA 2:Plasmid DNA after restricting enzyme

MonoFas Ⅰ (Extracted Fragment DNA from Agarose Gel)

Condition:Cycle sequencing by the Big Dye Terminator v3.1 manufactured by ABI

DNA sequencer:ABI 3730 Genetic Analyzer

Wide Adaptive Range of Fragment DNA Sizes

Due to the strong retention ability for nucleic acid, MonoFas I can effectively purify the fragment DNA in wide range from 35bp to 35 kb

Recovery Rate by Elution Volume and DNA Size

Elution Volume	Recovery Rate
5µL	83%
10µL	89%
15µL	91%
25µL	88%
50 µL	83%

Recovery rate was examined with the 400bp fragment DNA.

DNA Size	Recovery Rate
35bp	65%
80bp	80%
100bp	85%
500bp	93%
1000bp	96%
2000bp	94%
5000bp	92%
10000bp	96%
15000bp	96%
20000bp	95%
30000bp	92%

MonoFas I can be operated with a desktop centrifuge!
	Easy with the Desktop Centrifuge -Steady Rotation < 6,200 rpm (±20%)(Fixed) -Constant Rotation < 2,000xg (19,6000)m/s²
The recovery rate does not change depending on the revolution speed or revolution time.
●Fragment DNA : 35bp	●Fragment DNA : 35kb

Fragment size from 35bp to 35kb can be purified by spinning down. However, for the washing process, it needs an 1 minute centrifuge.

Items	Volume	Cat.No
MonoFas DNA Purification Kit Ⅰ50 pcs.	50pcs.	5010-21530
MonoFas DNA Purification Kit Ⅰ 100 pcs.	100pcs.	5010-21531
MonoFas DNA Purification Kit Ⅰ 250 pcs.	250pcs.	5010-21532
BufferA for MonoFas DNA Purification Kit Ⅰ	50mL	5010-21506
BufferB for MonoFas DNA Purification Kit Ⅰ	21mL	5010-21509
BufferC for MonoFas DNA Purification Kit Ⅰ	10mL	5010-21508

"Based on monolithic technology, Merck KGaA, Darmstadt.Germany""