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MonoTip Trypsin is a sample preparation tip with Silica Monolith that consists
continuous of through-pores with protein digestive enzyme, Trypsin, fixed.
Trypsin Digestion is Available only by Pipetting
Existing Trypsin digestion required a long time period to digest at 37℃
with a low concentrated Trypsin solution to avoid the self-digestion of
Trypsin. However, MonoTip Trypsin digests only by a simple pipetting operation
without a thermostatic chamber.
Only 20 minutes to Digest!
At 37º, it usually takes more than 10 hours to digest proteins using Trypsin
enzyme solution. With MonoTip Trypsin, it only takes a 2-3 minute pipetting
operation to digest under room temperature for 20 minutes.
High Enzyme Activity
With a large surface area of Silica Monolith, MonoTip Trypsin has 100ug
Trypsin fixed per tip.
This enables a quick digestion of a highly concentrated protein (See picture
right) MonoTip Trypsin has Trypsin derived from cow pancreas.
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| Items |
Specification |
| Time |
20 minutes |
| Sample Volume |
20~200µL |
| Tip Volume |
200µL |
| Derivation |
Cow Pancreas |
| Organic Solvent Resistance |
Acetonitrile less than 20% |
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| Items |
Specification |
| Monolith |
Highly Pure Sol Gel
Silica Gel |
| Through-pore Diameter |
10~20µm |
| Meso-pore Diameter |
30nm |
| Surface Area |
100m2/g |
| Chemical Bonding |
TPCK treated Trypsin |
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| <Condition> |
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<Condition> |
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| Column |
Inertsil WP300 C8
(150 x 4.6 mm.I.D.) |
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Column |
Inertsil WP300 C8
(150 x 4.6mm I.D.) |
| Eluent |
A:H2O(0.1% TFA) B:CH3CN(0.1% TFA)
A/B=90/10-(10min)-40/60 |
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Eluent |
A: H2O(0.1%TFA) B:CH3CN(0.1%TFA)
A/B=90/10-(20min)-40/60 |
| Flow rate |
1mL/min |
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Flow rate |
1 mL/min |
| Detection |
UV 210nm |
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Detection |
Detector : UV 210nm |
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As Trypsin is fixed to the Silica Monolith, Trypsin itself does not touch each other. As there is no self-digestion
of Trypsin, dramatic high protein purification can be achieved.
Also the fixed Trypsin is TPCK treated to inactivate the chymotrypsin activation.
Look!
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Long Life Time Enzyme Activity !
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MonoTip Trypsin can be stored under room temperature for 2 weeks, and in
a refrigerator for 1 year. Due to the long preservation term, there is
no concern to use 96 pcs, of MonoTip Trypsin in a long run.
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The following chromatography shows the analysis result of β-casein, Bovine Serum albumin and transferrin after treated with MonoTip Trypsin
by LC-MS.
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| Items |
Cat.No |
| MonoTip Trypsin (24 pcs) |
5010-21012 |
| MonoTip Trypsin (96 pcs) |
5010-21010 |
"Based on monolithic technology, Merck KGaA, Darmstadt, Germany"
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