Monolithic Capillary HPLC Columns
GL Sciences’ MonoCap series, created synthetically via sol-gel method, and an octadecyl silane chemically bonded, has a very uniform three dimensional structure that shows excellent reproducibility from batch-to-batch. The solid structure of GL Sciences’ monolithic silica eliminates the
need for frits or filters at the ends of the column, thereby reducing dead volume that might otherwise lead to band broadening or sample recovery. The high porosity of our monolithic silica allows high flow rates to be used without loss of resolutionor creation of high operating pressure. An optimized balance of throughpores and mesopores provides the critically importantcombination of efficiency, separation speed, large volume sample-loading, and small volume sample-recovery.
MonoCap High Resolution provide extremely high efficiency, delivering over 200,000 plates for a 2,000 mm length column. The High Resolution Ultra type deliver over 300,000 plates. The Fast-flow type is compatible with high flow rate analysis due to its’ low flow resistance. In addition, the equilibration time can be minimized further by setting the flow rate high.
MonoCap Nano-flow is a high-density monolithic capillary column offering extremely high sensitivity in LC/MS due to the optimization
of mesopore and throughpore sizes. MonoCap Trap columns have a relatively big throughpore, which are available for on-line preconcentration or desalting of protein and peptide samples prior to HPLC separation with mass spectrometry detection.
MonoCap Series Brochure
Physical Properties of MonoCap Series
|Description||Monolithic Silica||Skeleton||Throughpore||Mesopore||Porosity||Bonded Phase||End-capping||Max. Operating Pressure|
|MonoCap C18 High Resolution 750||High Purity Silica Gel||1 μm||2 μm||15 nm||85%||Octadecyl Groups||Yes||22 MPa|
|MonoCap C18 High Resolution 2000||1 μm||2 μm||15 nm||85%||Octadecyl Groups||Yes||35 MPa|
|MonoCap C18 High Resolution Ultra 2000||1 μm||2 μm||11 nm||85%||Octadecyl Groups||Yes||35 MPa|
|MonoCap C18 Fast-flow||1 μm||2 μm||15 nm||85%||Octadecyl Groups||Yes||22 MPa|
|MonoCap C18 Nano-flow||1 μm||1 μm||11 nm||85%||Octadecyl Groups||Yes||22 MPa|
|MonoCap C18 WideBore||1 μm||2 μm||11 nm||85%||Octadecyl Groups||Yes||22 MPa|
|MonoCap C18 Trap Column||2 μm||5 μm||11 nm||85%||Octadecyl Groups||Yes||20 MPa|
|MonoCap Amide||1 μm||2 μm||15 nm||85%||Carbamoyl Groups||None||22 MPa|
|MonoCap SCX||2 μm||5 μm||11 nm||85%||Benzenesulfonyl Groups||None||20 MPa|
|MonoCap HILIC-UP High Resolution 2000||1 μm||2 μm||12 nm||85%||Ureidopropyl Groups||None||35 MPa|