Titansphere™ TiO Bulk Material
Bulk Sorbent Materials for Purification & Enrichment of Phosphopeptides
While GL Sciences’ Phos-TiO spin columns and MonoTip TiO pipette tip based enrichment products are useful for most sample pretreatment applications, some investigators require bulk titanium dioxide material for specialized applications. Titansphere TiO bulk sorbent is available in 5µm and 10µm particle sizes in quantities of 500mg.
Purification / Enrichment Protocol
Spherically shaped porous particle “Titansphere TiO” (Titanium Dioxide ; TiO2) is a high-performing bulk material to enrich and purify phosphopeptides selectively.
- Efficient purification from HeLa Cell Lysate
The graph below shows the superior performance of Titansphere TiO using the HeLa Cell Lysate consisting mainly of non-phosphorylated peptides. Titansphere TiO shows exceptional selectivity – almost 90% of the bound peptides were phosphopeptides, and excellent capacity for total phosphopeptide binding. Whereas a competitive TiO product binds mainly non-phosphorylated peptides and a much lower total number of discrete phosphopeptides.
Sample: HeLa Cell Lysate, Sample volume: 50 μg, Titansphere TiO volume: 1 mg
- Comparison of Titansphere TiO and IMAC
The graph below shows the comparison of Titansphere TiO and IMAC enrichment using Arabidopsis cell extract. Titansphere TiO provides substantially higher total capacity and a much higher number of isolated phosphopeptides.
Sample: Arabidopsis Cell Extract, Sample volume: 100 μg, Titansphere TiO volume: 1 mg
- Example of how to make a Titanium Column
◊ Cut the Empore Disk C8 and put it at the tip of the 200ul pipette tip to be used as a filter.
◊ Stir and suspend the Titansphere TiO atCH3CN/H2O=80/20.v/v then load it to the tip.
(Adjust the amounf of the beads depending on the purpose)
◊ Pressure the tip by a plastic syringe.
|Particle Size||5 µm, 10 µm|
|Adsorption Spot||Titanium Dioxide Crystal|
|Pore Size||100 Å (10 µm)|
|pH Range||2 - 12|
Identified Numbers of Phosphopeptides
|Phosphorylate||Non - Phosphorylate|
Titansphere™ TiO Part Number
|Titansphere TiO 5 µm||500 mg||5020-75000|
|Titansphere TiO 10 µm||500 mg||5020-75010|
|* Titansphere Phos-TiO Bulk 10 um||500 mg||5010-21315|
* New material for higher purification efficiency of Phosphopeptides
Empore Disk C8 Part Number
|Empore Disk C8, 47 mmDIA, 0.5 mm Thickness||20 pcs||5010-30002|
|Empore Disk C8, 90 mmDIA, 0.5 mm Thickness||10 pcs||5010-30003|