GL-Tip SCX and GL-Tip SDB-SCX

GL-Tip SCX and GL-Tip SDB-SCX

 

Spin tips for peptide fractionation

GL-Tip SCX is packed with strong cation polymer (SCX) and GL-Tip SDB-SCX are packed with styrene divinylbenzene polymer (SDB) and strong cation polymer (SCX). GL-Tip SDB-SCX is packed in a two layer format consisting an SDB and SCX media. Undesalted peptide samples can be used in GL-Tip SDB-SCX as the first SDB layer can desalt the sample.

 

Sample loading capacity

sample-loading-capacity

 

 

TFA gradient elution using phosphopeptides derived from DLD-1 human large intestinal cancer cells

tfa-vs-traditional-gradient-elution

 

 

 

Number of Quantified Peptides using GL-Tip SCX

scx-sdb-scx-data

Compared results between commercially available brand G’s tip column. GL-Tip SCX recovered more peptides. The usage of the newly developed TFA gradient elution method provide less chance of identifying the same peptide from one fractionated sample peptide to another resulting in higher efficiency.

 

Ordering Information

DescriptionTip VolumeQty.Cat. No.
GL-Tip SCX200 μL96 pcs7510-11203
GL-Tip SDB-SCX200 μL96 pcs7510-11202
Centrifuge Adapter24 pcs5010-21514
Centrifuge Adapter for 96-Well Plate1 /pk5010-21341
Centrifuge Adapter for 96-Well Plate2 pcs5010-21343