Conference & Exhibition

Proteomics LC/MS: How to Maximize Sensitivity, Depth of Analysis, Throughput and Robustness

In bottom-up proteomics, the key is how to efficiently separate extremely complex peptide samples that contain more than a million types of peptides in a concentration range of more than six orders of magnitude. For this purpose, nano-scale liquid chromatography (LC) and tandem mass spectrometry (MS) are used, in which precursor ions are separated by both reverse-phase LC using an acidic mobile phase and a front-mass analyzer such as a quadrupole. Then, collision-induced dissociation is used to generate the series of product ions necessary for peptide identification. This webinar will discuss the optimal proteomics LC/MS that produces a synergistic effect between LC and MS, in contrast to chromatographers who only think of MS as a detector and mass spectrometrists who try to solve everything with MS alone. It will introduce a new system that achieves a balance between depth of analysis, sensitivity and throughput, and lastly, system robustness, which is always a problem in actual analysis.

Key Learning Objectives:
・Learn how LC and MS work for efficient peptide separation in proteomics.
・Discover a system that balances analysis depth, sensitivity, and throughput.
・Understand strategies to improve system robustness in proteomics analysis.