Nutrition

The most commonly used type of sugar analysis is a post-column system that separates columns for subsequent fluorescence derivatization. It has the advantage that it is not only sensitive and analytical robustness, but also selectivity, and is less susceptible to contaminants.

The combination of HPLC RI and NH₂ columns, which are not sensitive but can be easily assayed for sugar, is low widely used. Normally, 4.6 mm internal diameter column is used, but changing to an internal diameter 3.0 mm column allows the mobile phase to be reduced by 60 % while maintaining separation patterns.

Electrochemical detectors (ECDs) can detect sugars with high sensitivity without derivatization.

Electrochemical detectors (ECDs) can also detect sugar alcohols that are difficult to measure with post-column derivatization methods.

The most common amino acid analysis system is a post-column fluorescence derivatization system. Superior sensitivity, selectivity, and method robustness.

Precolumn derivatization, which is derivatization prior to injection into the HPLC, can be assayed without the availability of pumps or reaction units to deliver derivatization reagents. This application is an example of measurements using two derivatization reagents, OPA and FMOC.

Example of measurement using NBD-F derivatization reagent.

When analyzing highly polar amino acids without derivatization, HILIC modes should be used. Since metal coordination adsorption is sometimes observed, it is recommended to use a metal-free tank for high-sensitivity analysis.

In the analysis of organic acids, many post-column systems are used to increase the selectivity of detection by using the color change of BTB reagent only when the desired components are eluted from the column.

This application also uses BTB method, but the separation conditions are different from "1". This is used when the peak of the desired ingredient overlaps with contaminants under the conditions of "1".

This is an example of the measurement of components used for the calculation of the fish freshness determination constant (K-value)... InertSustain AQ C18 can be retained and separated without the use of ion-pairing reagents.

Although some catechins are not sensitive to UV detectors, electrochemical detectors (ECDs) can be used to detect and quantify 10 components with high sensitivity.

Thiamin separated on ODS columns reacts with potassium ferricyanide under alkaline conditions to produce the fluorescent thiochrome, which can be selectively and sensitively detected using a fluorescence detector.

Because riboflavin is a naturally fluorescent substance, it can be selectively and sensitively detected using a fluorescence detector after separation on an ODS column.