Metabolomics is a comprehensive qualitative and quantitative analysis of low-molecular-weight metabolites in living organisms. Measurements are mainly made using a mass spectrometer. Because metabolites have a direct effect on phenotype compared to genes and proteins, research has become popular in recent years, and metabolomic research is expected in fields such as disease diagnosis.
GC/MS is capable of comprehensively measuring metabolites by derivatization. It is also characterized by the availability of libraries that make it easier to approach qualitative.
- ~GC/MS Metabolomics Library for Efficient and more Accurate Qualitative~
- Example: for miso, sake and soy sauce
Column for GC/MS Analysis
A low polar column chemically bonded with 5 % diphenyl-95 % dimethylpolysiloxane realizes high inertness and low bleed.
Mixed Metabolite Samples for Metabolomics
Checking the reaction efficiency of derivatization and the operation status of the instrument before measuring real samples is important for obtaining stable data. This mixed sample can be measured at regular intervals for each measurement of the actual sample and analyzed statistically to detect fluctuations in the analytical method and contamination and abnormalities of the equipment.
LC/MS analysis is used for target analysis. Select a column for your target compounds.
- Analytes of Metabolites by LC/MS/MS ~ Amino Acids ~
- Examples of Analyses of Nucleotides in Fish Meat
Columns for LC and LC/MS Analysis
InertSustain C18 are highly inert ODS columns. Amino acids and nucleic acids can be analyzed using ion-paired reagents.
InertSustain Amide are HILIC columns with hydrophilic interactions. Metabolites can be separated without the use of ion pair reagents.
In addition, ATP with a phosphate group, etc., exhibits metal coordination adsorption. Metal-free columns that eliminate metal in the liquid contact section are expected to improve peak shape and S/N.
Desalting prior to sample introduction into the LC/MS is an essential process for protecting the equipment. The MonoSpin C18 is easily desalted by centrifugation.