There are several methods with GC to analyze metabolites, such as amino acids, organic acids, fatty acids, sugars, and so on. Methoximation and trimethylsilylation (TMS) is the classical and most widely used derivatization procedure for metebolome analysis with GC.
In this note, the about 400 metabolites widely used TMS derivatized were detected with GC-MS (the column was InertCap 5MS/NP).