Glyphosate (n-(phosphonomethyl)-glycine) is an effective herbicide in killing a wide variety of plants. This note describes a determination method for glyphsoate using an HPLC system.
In Japan, 2 mg/L is set as the target value and two determination methods are described in an ordinance from Ministry of Health,
Labour, and Welfare concerning water quality standard for tap water. In conformity with one of them, 9-fluorenylmethyloxy-carbonyl chloride (FMOC) was used as a pre-column derivatization reagent in this note.
As well as glyphosate, aminomethylphosphonic acid (AMPA), which is its major metabolite, should be simulatneously determined because it is described in the ordinance that concentration of AMPA has to be added to that of glyphosate.
In the quality control of Japanese pesticide analysis, the coefficient of variation (CV) at one-hundredth concentration of the target value is required to be less than 20 %. In this note, CV values even at one-thousandth concentration of the target value were examined.