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Analysis of Scopolamine and Hyoscyamine in Scopolia Extract in Accordance with the Japanese Pharmacopoeia
Scopolia extract powder is derived from Scopolia japonica Maxi. It is a crude drug prepared by drying the roots and extracting the active ingredients. It contains approximately 0.1 % scopolamine and hyoscyamine in the dried product (atropine in this racemic mixture). It is used in combination with over-the-counter stomach drugs as an antispasmodic and analgesic because it reduces the secretion of stomach juice and excessive motility of the gastrointestinal tract, and prevents the transmission of pain.
The Japanese Pharmacopoeia employs an HPLC method with ODS-column for the determination of scopolamine and hyoscyamine in scopolamine extract powder. The following system suitability items are specified.
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Compounds
- Scopolamine Hydrobromide
- Scopolamine
- Atropine Sulfate
- Brucine
- Hyoscyamine Sulfate Hydrate
Keywords
C18|ODS|UV|Inertsil ODS-SP