This note describes a determination method of hydrogen peroxide (H₂O₂) using HPLC-ECD (high- performance liquid chromatography-electrochemical detection) system.
H₂O₂ is used for various purpose, such as disinfectant, oxidizing agent, and rinse solution. Determination of H₂O₂ is required also for evaluation of fuel cells. Simple determination method for H₂O₂ was often performed by titration or voltammetry. However, the detection of these methods lack selectivity.
ECD, which is similar to voltammetry in principle, detects electric current generated by applied oxidation or reduction potential. However, the detection by ECD is performed after the HPLC separation, which provides excellent selectivity.
The method described in this note enables sensitive detection and accurate quantification of H₂O₂.