Switching the Mobile Phase from Acetonitrile to Methanol
Today, the supply shortage of acetonitrile, which is one of the most widely used organic solvents in HPLC analysis, became extremely hot topic. Raw acetonitrile is obtained as a co-product of acrylonitrile, which is used in automobile component. As a consequence of the global economic recession and the associated reduction of demand for acrylonitrile, there has been a serious decrease in the supply of acetonitrile.
Methanol, which is also widely used as mobile phase, is easily available at a low price. In this note, how to use methanol instead of acetonitrile is described. What should be paid attention to are elution strength, elution order, and back pressure.