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Improvement of purification using HPLC column -Comparison between column chromatography and preparative HPLC-
To purify crude product of organic synthesis or to isolate some compounds from natural product, column chromatography with silica gel of 50 ?m - 200 ?m particle is generally used. However, separation efficiency can be improved by use of 5 ?m - 10 ?m particle for HPLC column. Moreover, separating time can be reduced because optimal flow rate for 5 ?m - 10 ?m particle is faster than that for 50 ?m - 200 ?m particle.
We recommend purification using HPLC when sufficient performance is not offered with conventional column chromatography or flash column chromatography.
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Compounds
- p-Hydroxy benzoic acid isobutyl ester
- p-Hydroxy benzoic acid n-butyl ester
- Butyl p-Hydroxybenzoatesec-
Keywords
C18|ODS|UV|Inertsil ODS-3