To purify crude product of organic synthesis or to isolate some compounds from natural product, column chromatography with silica gel of 50 ?m - 200 ?m particle is generally used. However, separation efficiency can be improved by use of 5 ?m - 10 ?m particle for HPLC column. Moreover, separating time can be reduced because optimal flow rate for 5 ?m - 10 ?m particle is faster than that for 50 ?m - 200 ?m particle.
We recommend purification using HPLC when sufficient performance is not offered with conventional column chromatography or flash column chromatography.