It became a serious problem in China and Taiwan in 2013 that cyanoguanidine, or dicyandiamide, was detected in powdered milk made in New Zealand. Since cyanoguanidine is contained in some chemical fertilizer as nitrification inhibitor, it is considered as a possible cause of the problem that such fertilizer was used in ranch.
In 2008, melamine was detected in milk and dairy products, which also became a big issue. HILIC (hydrophilic interaction chromatography) mode was used for the determination of melamine content
because melamine is highly hydrophilic.
Chemical structure of cyanoguanidine is similar to that of melamine. In this note, cyanoguanidine was analyzed with HILIC mode. Cyanoguanidine and melamine could be determined simultaneously using Inertsil HILIC as a separation column.