Takadiastase (Aspergillus oryzae carboxyl proteinase) has been used for hydrolyses of thiamin phosphate ester and riboflavin phosphate ester to analyse thiamin and riboflavin, respectively by HPLC.
However, it is difficult to get commercial Takadiastase stable nowadays.
So in this technical note, we introduce a new sample preparation method for thiamin and riboflavin analyses using acid phosphatase hydrolysis instead of Takadiastase hydrolysis.