Ribavirin is an anti-viral medication used in combination with Interferon to treat Hepatitis C. Since it easily accumulates in human body, it is possible to forecast and study the therapeutic effects by pharmacokinetic analysis.
As Ribavirin is a hydrophilic drug, sample preparation by hydrophobic interaction using phases such as C18 spin will most likely not work. For this reason in the note below a MonoSpin PBA column, which shows selective adsorption for cis-diols contained in Ribavirin, was used for purification.
Ribavirin was added to 0.25 ? 25 μg/mL of human serum which was then purified by using MonoSpin PBA and analyzed by HPLC.
The analytical column used is InertSustain AQ-C18 which provides stability even under highly aqueous mobile phase conditions.
The results show that the measurement of Ribavirin in Human Serum was conducted in an easy and selective way.
In this note other remarks are described which may help you developing your method.