Organic acids are used as sour flavorings, antioxidants, and antibacterial agents in foods, and their properties are attracting significant attention. In this report, InertSphere FA-1 was used in the analysis of organic acids and separation was made using ion exclusion mode; which is the most commonly used method for organic acid analysis. The sulfonic acid group on the column repels organic acids ionically and elution is made in order from the smallest pKa.
In addition, a post-column method using BTB was used, which has high selectivity and is resistant to contaminantants. A simple pretreatment method with detection using a visible absorption wavelength (440 nm) offers high-precision analysis with fewer interfering peaks due to the presence of contaminants.