Since preparative HPLC uses a large amount of solvent, it is important to keep the temperature of the mobile phase (eluent) uniform. Analytical columns are generally temperature controlled, but preparative columns are sometimes kept at room temperature without temperature control.
Even with a preparative column, a constant retention time leads to accurate preparative separation, so it can be said that temperature control of the preparative column is particularly beneficial when performing repeated preparative separations.
This time, following technical notes No. 133 and No. 134, we will introduce the following points for column temperature control as "How to use preparative separation well, Part 3".