Fumonisins B1 and B2 are mycotoxins found in cereals and are typically analyzed using HPLC with fluorescence detection or LC/MS (/MS). Sample pretreatment, is commonly made using a strong anion exchange phase (SAX) because fumonisin has several carboxylic acids in its molecular structure. However, extraction with SAX alone may result in low recoveries for the samples with a high lipid content or a complex composition e.g.beer. These sample types also require pretreatment to remove lipids and other contaminants with non-polar phases such as C18 prior to treatment with the SAX phase. Two extraction methods are introduced in this application note , the first using SAX only, and the second using C18 and SAX in tandem.