HPLC Basic Knowledge Table of Contents HPLC Column Technical Guide 1. How to Use Buffers 2. Organic Solvents for Mobile Phases 3. How to Replace a Non‐aqueous Solvent with an Aqueous Solvent 4. Tubing Connection 5. Supplementary Information on Units Used in HPLC 6. Basic Chromatographic Terms How to Use Analytical Columns 1. How to Obtain Good Peak Shapes 2. How to Change Elution Patterns 3. How to Increase Retention 4. How to Achieve Fast Elution 5. How to Improve Sensitivity 6. How to Prolong Column Lifetime 7. How to Improve the Robustness of Your Methods 8. Downscaling on a Conventional HPLC System 9. How to Shorten Analysis Time on a Conventional HPLC system 10. Monolithic Silica Columns 11. Organic Solvents Used in HPLC 12. Materials of the Wetted Parts 13. Analyte Adsorption in Ion-exchange Mode How to Use Preparative HPLC 1. Sample Solution 2. Column Selection 3. Upscaling 4. Fractionation with an Ion-paring Reagent 5. Temperature Difference Between Column and Mobile Phase 6. Recycling Preparative HPLC
