Panipenem is an antibiotic that is active against a variety of bacteria, including Streptococcus pneumoniae, Escherichia coli, and Pseudomonas aeruginosa. Indications include sepsis, pneumonia, osteomyelitis, and sinusitis.

In this application, a water test as described in the 16th edition of the Japanese Pharmacopoeia was performed using a packed column. Excellent results were achieved in terms of both separation and reproducibility for water and acetonitrile in methanol.