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Analysis of Water-soluble Vitamins by HPLC-ECD
This note describes a determination method for water-soluble vitamins using an electrochemical detector (ECD).
Analysis of water-soluble vitamins are generally performed using UV detection coupled with reversed-phase HPLC. However, sensitive determination of some vitamins [e.g. biotin (Vitamin B7)] with HPLC-UV system is difficult, because their response to UV detector is very weak. In such case, ECD is a powerful alternative to UV detector. Biotin can be detected with high sensitivity as well as cyanocobalamin because both are electrochemically reactive species.
ED703 pulse is an ECD equipped with boron-doped diamond electrode. The diamond working electrode offers stable response, low background current and wide potential window. ED703 pulse is very useful to quantify various compounds.
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Compounds
- Biotin
- Ascorbic acid (Vitamin C)
- Pyridoxine (Vitamin B6)
- Pyridoxal (Vitamin B6)
- Thiamine (Vitamin B1)
- Cyanocobalamin (Vitamin B12)
- Pyridoxamine (Vitamin B6)
Keywords
C18|ODS|Vitamin|ECD|Inertsil ODS-SP|Inertsil ODS-3