This note describes a simultaneous determination method for 9 phenolic antioxidants using a binary gradient HPLC system.
Among them, propyl gallate (PG), nordihydro-guaiaretic acid (NDGA), butylated hydroxyanisole
(BHA), and butylated hydroxytoluene (BHT) are permitted as food additive in Japan. Meanwhile, 2,4,5-trihydroxybutyrophenone (THBP), tert-butylhydro-quinone (TBHQ), 4-hydroxymethyl-2,6-di-tert-butyl-phenol (HMBP), octyl gallate (OG), and dodecyl gallate (DG) are used in other countries.
All of the phenolic antioxidants can be determined with a photodiode array (PDA) detector. Fluorescence detection
also can be utilized except for THBP and HMBP. In this note, a PDA detector and a fluorescence detector were connected in series, and the chromatograms obtained with both detectors were shown.