This note describes a determination method for phenolic antioxidants using an Inertsil Ph-3 column, in which phenyl groups chemically bonded directly to porous silica particles.
In a previous note (No.64), sufficient separation of the antioxidants was achieved by an ODS column coupled with gradient elution of mobile phase. However, if an interfering peak is detected near an analyte peak or an unknown peak is required to be identified, it is necessary to use another column in which stationary phase is modified with different functional groups.
In this note, Inertsil Ph-3 was chosen among reversed-phase HPLC columns. As well as good separation of the antioxidants was obtained, elution order was significantly changed owing to the interaction between π electrons of the benzene rings bonded to the column particles and the aromatic analytes.