We present a preprocessing method using MonoSpin SCX and propranolol hydrochloride analyses in biological samples using a laser-excited fluorescent detector LIF726.
Propranolol hydrochloride is a β-blocker that is used to treat angina, arrhythmias, and high blood pressure. This is one of the drugs that require blood monitoring because of the close therapeutic and poisoning range of blood concentrations, in addition to the large individual differences in drug metabolism. The therapeutic range is determined to be 10 μg/L to 100 μg/L in plasma, which requires a highly sensitive detection method. The laser-excited fluorescence detector used here enables sensitive and selective detection by using a laser as the source of the fluorescence detector.
10 n pages, we added propranolol hydrochloride to sera, then deproteinized them with organic solvents, and measured them HPLC. On p. 3, plasma was pretreated with propranolol hydrochloride followed by MonoSpin SCX and analyzed.