When analyzing glycans, Fluorescent derivatization method is often used before measurement by HPLC. Although large amounts of unreacted derivatizing reagents remain in the solution immediately after the derivatization reaction, only unreacted reagents can be removed by performing solid-phase extractions prior to injecting them into the HPLC.
Using MonoSpin, a spin-type solid-state column, separate sugar chains derivatized with 2-aminobenzamide (2-Aminobe?nzamide,2-?AB)? from unreacted reagents. As a result of evaluating the two types of MonoSpin Amide and MonoSpin NH2, both of them recover sugar chains of more than trisaccharide.