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3. How to Replace a Non‐aqueous Solvent with an Aqueous Solvent

Normal‐phase columns of InertSustain and Inertsil are filled with a non‐aqueous solvent. When using these columns in reversed phase mode, replace the solvent by following the procedure shown in Figure 1. Especially if the mobile phase contains a salt, please avoid salt precipitation to prevent damage to the column. 2‐propanol (IPA)and ethanol are typical intermediate solvents and alcohols generate a relatively high column pressure. Please be careful of the maximum operating pressure of the column and adjust the flow rate if necessary.