In Japan, Agency for Natural Resources and Energy is considering to amend the law “Enforcement Regulation of controlling the quality of volatile oils”, which is to strictly control the quality of biodiesel fuels (BDF).
As a result, a regulation of the compounds shown in the right table has been added. FAME (Fatty acid methyl esters) and TG (Triglycerides) in BDF are required to be analyzed using the HPLC method. Concentrations of FAME and TG are obtained from their calibration curves that have been already prepared using methyl stearate and trilinolein as their standard, respectively.