Antibody Purification Columns
Protein L is a protein which has an affinity for the variable region of the immunoglobulin kappa light chain (VL). The ligand of MonoSpin ProL is the Protein L used for KANEKA KanCapTM L. MonoSpin ProL enables purification of antibodies including kappa light chains or their fragments such as scFv, Fab and F(ab')2.
Rapid purification and high recovery of antibodies
Antibody Loading Capacity
Centrifuge MonoSpin ProL attached to a 1.7 mL or 2.0 mL microtube.
Selectivity of MonoSpin ProL
Purification of Fab by MonoSpin ProL
The Fab was purified from CHO cell culture fluid by MonoSpin ProL. The HPLC results before and after purification were compared using MonoSelect RP-mAb as the analytical column. This comparison and the result of SDS-PAGE confirm purification of Fab by MonoSpin ProL.
Column: MonoSelect RP-mAb
Injection Vol.： 1μL
Purification of papain-digested Fab by MonoSpin ProL
Papain digestion for monoclonal antibodies purified from CHO cell culture by MonoSpin ProA separates the monoclonal antibodies into Fab and Fc regions. Then the separated Fab can be recovered and purified by MonoSpin ProL.
|MonoSpin ProL Column Type