MonoTip C18 pipette tip contains silica monolith bonded with C18 (Octadecyl groups). Silica monolith has continuous through-pore structure. As C18 is bonded, MonoTip C18 can desalt and concentrate proteins and peptides in reversed phase mode by easy pipetting.
How to Use Mono Tip
With MonoTip C18, samples are processed by pipetting. The operation is easier and less time consuming than using conventional SPE methods
(Refer to applications for wide variety molecular weight compounds as below.)
High efficiency with a wide range of molecular weight
Continuous through-pore is the structural advantage of silica monolith, which enables MonoTip C18 to offer high efficiency. MonoTip C18 is available for peptide and protein samples with the concentration range from pmol to nmol
A MonoTip C18 with a tip volume of 200 μL corresponding to a large volume sample and a MonoTip mini C18 with a volume of 10 μL corresponding to a trace sample were prepared.
Adapted to Peptide-Protein Samples with a Molecular Weight of Up to 40,000 in the Sample Concentration pico mol to nano molar order
Purifications of antihistamine in serum
Hasegawa C et al., Rapid Commun. Mass Spectrom (2006);20:537-543
“Simultaneous determination of ten antihistamine drugs in human plasma using pipette tip solid-phase extraction and gas chromatography / mass spectrometry”
Purification of sulfatide (sulfoglycosphingolipid)
Kyogashima M et al., Glycobiology (2006); 16(8),719-728
“Rapid demonstration of diversity of sulfatide molecular species from biological materials by MALDI-TOF / MS