Capillary HPLC Columns

MonoCap Amide

Amide groups are chemically bonded to the monolithic silica and makes it suitable for the analysis of sugars via HILIC mode. As the back pressure is significantly low, a 500 mm length MonoCap Amide column deliver over 40,000 plates offering high efficiency. Generally, HILIC mode uses acetonitrile at a concentration between 65 % - 95 % in an aqueous buffer such as ammonium acetate or ammonium format, which have high solubility in organic solvents. Columns are protected by either metal or PEEK hardware.

Figure 1. Analysis of Sugar Alcohol and Oligosaccharide

Figure 2. Effect of Concentration of Organic Solvent on Retentivity

Figure 3. Effect of Concentration of Salt on Retentivity

Figure 4. Analysis of Pyridylaminated Sugar Chain